Principles of Cloning, Third Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species - including mice, sheep, cattle, and non-mammals is considered as well. The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine - Dr John Gurdon; the cloning of the first mammal from a somatic cell - Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual - Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell - Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas.

Table of Contents:
1. Cloning History and Universal Principles Part I Basics 2. Multiplying Embryos: Experimental Monozygotic Polyembryony in Mammals and its uses 3. Artificial Activation of Mammalian Oocytes 4. Nuclear Origins and Clone Phenotype 5. Genetic and Phenotypic Similarity Among Members of Mammalian Clonal Sets Part II Methods 6. Micromanipulation Techniques for Cloning 7. Handmade Cloning 8. Culture of Viable Mammalian Embryos In Vitro 9. Pregnancy and Neonatal Care of SCNT Animals 10. Donor Cell Type and Cloning Efficiency in Mammals 11. Enhancing SCNT with Chromatin Remodeling Agents 12. Cell Cycle Regulation in Cloning Part III Cloning by Species 13. Amphibia 14. Medaka Fish 15. Zebrafish 16. Goldfish 17. Mice 18. Rabbits 19. Pigs 20. Cows 21. Dogs 22. Equids 23. Macaque Monkeys Part IV Complementary Technologies 24. Genome Editing in Somatic Cells 25. Targeted Chromosome Elimination from ES-Somatic Hybrid Cells Part V Applications 26. Nuclear Transfer for Stem Cells 27. Embryonic Stem Cell in Bovine (other species?) 28. Commercial Applications of Cloning Technology 29. Production of Recombinant Therapeutic Proteins 30. Generation of Transgenic Domestic Species via Somatic Cell Nuclear Transfer 31. Commercializing Genetically Engineered Cloned Cattle 32. Cloning Endangered Species 33. Genetically Engineered Pigs for Medicine Part VI Mechanisms 34. Histone Methylation During Somatic Cell Nuclear Transfer 35. Nuclear Transfer with Germ Cells: Germ Cell Cloning Contributes to current Understanding of Genomic Imprinting in Mammals 36. Clone-Specific X-Linked Gene Repression Caused by Ectopic Xist Transcripts from the Active X Chromosome 37. Cell Division and Nuclear Reprogramming 38. Reprogramming Machinery of the Mouse Oocyte – Proteomic Analyses 39. Biological Age of Cloned Animals 40. Mitochondrial Inheritance in Cloned Animals 41. Interspecies Somatic Cell Nuclear Transfer 42. Epigenetics of Cloned Livestock Embryos and Offspring 43. Role of iPSC-Producing Factors in Pre-Implantation Embryos 44. Chromatin architecture reorganization in murine somatic cell nuclear transfer embryos 45. Genome Exchange in Human Oocytes Part VII Ethical, Regulatory and Legal Affairs 46. Ethical Implications of Reproductive Cloning 47. An Overview of the Regulatory Considerations for Animal Cloning in the USA 48. Regulation and safety considerations of somatic cell nuclear transfer-cloned farm animals and their offspring used for food production – the European Union perspective 49. Asian Regulatory Landscape on Animal Cloning 50. South American Governmental guidelines for the production and commercialization of Cloned Animals Part VIII Cloning Outlook 51. Somatic cell nuclear transfer: failures, successes, and the challenges ahead